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| 11. |  | GRACE, N. D.; LEE, J. Effect of Co, Cu, Fe, Mn, Mo, Se, and Zn supplementation on the elemental content of soft tissues and bone in sheep grazing ryegrass/white clover pasture. New Zealand Journal of Agricultural Research, v.33, p.625-647, 1990.| Biblioteca(s): Embrapa Pantanal. |
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Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
07/10/2008 |
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Data da última atualização: |
07/10/2008 |
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Autoria: |
NIVA, C. C.; LEE, J. M.; MYOHARA, M. |
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Título: |
PCNA as a cell proliferation marker in the oligochaete Enchytraeus japonensis. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM. |
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Idioma: |
Inglês |
|
Conteúdo: |
PCNA (proliferating cell nuclear antigen) is a protein involved in DNA replication expressed in
dividing cells which has been used as a cell proliferation marker for clinical studies of neoplastic
tissues in vertebrates, and also for developmental studies in several vertebrates and
invertebrates. Among invertebrates, PCNA has been detected by immunohistochemical methods
in several organisms, including the fragmenting oligochaete annelid E. japonensis. Despite the
apparent positive cross-reaction with commercially available antibodies, it was unknown whether
PCNA protein was really synthesized by E. japonensis. The objective of this study was to clarify
this question and verify whether PCNA could be used as a reliable molecular marker for
proliferative cells during E. japonensis regeneration, growth and embryo development at the
RNA level. For that purpose we cloned a cDNA encoding PCNA from E. japonensis by carrying
out degenerate PCR with primers designed from the conserved regions of known PCNAs using
a cDNA library of regenerating E. japonensis as the template. Two full-length cDNA sequences
with identical deduced amino acid sequence were obtained and the amino acid sequence
showed high similarity to known PCNAs of other organisms. We analyzed the gene expression
by RT-PCR and in situ hybridization in whole mounts (WISH) and paraffin sections in intact and
regenerating worms. The results showed a strong expression of pcna gene in the developing
growth zone of intact worms. In regenerating fragments, pcna expression was first detected in a
few cells in the vicinity of the amputated surface, and then clearly seen in cell agglomerates in
the blastema as regeneration progressed. Embryos also showed strong expression. We conclude
that pcna is a good cell proliferation marker for E. japonensis and may be a good biomarker to
study effects of stressors on developmental processes. MenosPCNA (proliferating cell nuclear antigen) is a protein involved in DNA replication expressed in
dividing cells which has been used as a cell proliferation marker for clinical studies of neoplastic
tissues in vertebrates, and also for developmental studies in several vertebrates and
invertebrates. Among invertebrates, PCNA has been detected by immunohistochemical methods
in several organisms, including the fragmenting oligochaete annelid E. japonensis. Despite the
apparent positive cross-reaction with commercially available antibodies, it was unknown whether
PCNA protein was really synthesized by E. japonensis. The objective of this study was to clarify
this question and verify whether PCNA could be used as a reliable molecular marker for
proliferative cells during E. japonensis regeneration, growth and embryo development at the
RNA level. For that purpose we cloned a cDNA encoding PCNA from E. japonensis by carrying
out degenerate PCR with primers designed from the conserved regions of known PCNAs using
a cDNA library of regenerating E. japonensis as the template. Two full-length cDNA sequences
with identical deduced amino acid sequence were obtained and the amino acid sequence
showed high similarity to known PCNAs of other organisms. We analyzed the gene expression
by RT-PCR and in situ hybridization in whole mounts (WISH) and paraffin sections in intact and
regenerating worms. The results showed a strong expression of pcna gene in the developing
growth zone of intact worms... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 02578naa a2200145 a 4500
001 1315080
005 2008-10-07
008 2008 bl uuuu u00u1 u #d
100 1 $aNIVA, C. C.
245 $aPCNA as a cell proliferation marker in the oligochaete Enchytraeus japonensis.
260 $c2008
520 $aPCNA (proliferating cell nuclear antigen) is a protein involved in DNA replication expressed in dividing cells which has been used as a cell proliferation marker for clinical studies of neoplastic tissues in vertebrates, and also for developmental studies in several vertebrates and invertebrates. Among invertebrates, PCNA has been detected by immunohistochemical methods in several organisms, including the fragmenting oligochaete annelid E. japonensis. Despite the apparent positive cross-reaction with commercially available antibodies, it was unknown whether PCNA protein was really synthesized by E. japonensis. The objective of this study was to clarify this question and verify whether PCNA could be used as a reliable molecular marker for proliferative cells during E. japonensis regeneration, growth and embryo development at the RNA level. For that purpose we cloned a cDNA encoding PCNA from E. japonensis by carrying out degenerate PCR with primers designed from the conserved regions of known PCNAs using a cDNA library of regenerating E. japonensis as the template. Two full-length cDNA sequences with identical deduced amino acid sequence were obtained and the amino acid sequence showed high similarity to known PCNAs of other organisms. We analyzed the gene expression by RT-PCR and in situ hybridization in whole mounts (WISH) and paraffin sections in intact and regenerating worms. The results showed a strong expression of pcna gene in the developing growth zone of intact worms. In regenerating fragments, pcna expression was first detected in a few cells in the vicinity of the amputated surface, and then clearly seen in cell agglomerates in the blastema as regeneration progressed. Embryos also showed strong expression. We conclude that pcna is a good cell proliferation marker for E. japonensis and may be a good biomarker to study effects of stressors on developmental processes.
700 1 $aLEE, J. M.
700 1 $aMYOHARA, M.
773 $tIn: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM.
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